REGULATION OF NITROGEN-METABOLISM IS ALTERED IN A GLNB MUTANT STRAIN OF RHIZOBIUM-LEGUMINOSARUM

被引：30

作者：

AMAR, M ^{[1
]}

PATRIARCA, EJ ^{[1
]}

MANCO, G ^{[1
]}

BERNARD, P ^{[1
]}

RICCIO, A ^{[1
]}

LAMBERTI, A ^{[1
]}

DEFEZ, R ^{[1
]}

IACCARINO, M ^{[1
]}

机构：

[1] INT INST GENET & BIOPHYS, I-80125 NAPLES, ITALY

来源：

MOLECULAR MICROBIOLOGY | 1994年 / 11卷 / 04期

关键词：

D O I：

10.1111/j.1365-2958.1994.tb00346.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a Rhizobium leguminosarum mutant strain altered in the glnB gene. This event, which has never been described in the Rhizobiaceae, is rare in comparison to mutants isolated in the contiguous gene, glnA. The glnB mutation removes the glnBA promoter but in vivo does not prevent glnA expression from its own promoter, which is not nitrogen regulated. The glnB mutant strain does not grow on nitrate as a sole nitrogen source and it is Nod(+), Fix(+). Two -24/-12 promoters, for the glnII and glnSA genes, are constitutively expressed in the glnB mutant, while two -35/-10-like promoters for glnA and ntrBC are unaffected. We propose that the glnB gene product, the P-II protein, plays a negative role in the ability of NtrC to activate transcription from its target promoters and a positive role in the mechanism of nitrate utilization.

引用

页码：685 / 693

页数：9

共 40 条

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