URIDYLYLATION OF THE P(II) PROTEIN IN RHIZOBIUM-LEGUMINOSARUM

被引：22

作者：

COLONNAROMANO, S ^{[1
]}

PATRIARCA, EJ ^{[1
]}

AMAR, M ^{[1
]}

BERNARD, P ^{[1
]}

MANCO, G ^{[1
]}

LAMBERTI, A ^{[1
]}

IACCARINO, M ^{[1
]}

DEFEZ, R ^{[1
]}

机构：

[1] INT INST GENET & BIOPHYS, CNR, VIA MARCONI 10, I-80125 NAPLES, ITALY

来源：

FEBS LETTERS | 1993年 / 330卷 / 01期

关键词：

URIDYLYLATION; GS CASCADE; P(11) PROTEIN;

D O I：

10.1016/0014-5793(93)80927-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Permeabilization with cetyl trimethyl ammonium bromide was used to study the post-translational modification of the P(II) protein in Rhizobium leguminosarum. Upon incubation with radioactive UTP a single band was obtained after SDS-PAGE and autoradiography. RNase resistance and snake venom phosphodiesterase sensivity showed that radioactivity was bound through a phosphodiester bond to a protein which was absorbed by an antiserum specific for the P(II) protein. Uridylylation of the P(II) protein was shown to be dependent on the modifications of the glutamine/alpha-ketoglutarate ratio.

引用

页码：95 / 98

页数：4

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