DIRECT INTRODUCTION OF LARGE-VOLUME URINE SAMPLES INTO AN ONLINE IMMUNOAFFINITY SAMPLE PRETREATMENT CAPILLARY GAS-CHROMATOGRAPHY SYSTEM

被引:47
作者
FARJAM, A [1 ]
VREULS, JJ [1 ]
CUPPEN, WJGM [1 ]
BRINKMAN, UAT [1 ]
DEJONG, GJ [1 ]
机构
[1] FREE UNIV AMSTERDAM,DEPT ANALYT CHEM,DE BOELELAAN 1083,1081 HV AMSTERDAM,NETHERLANDS
关键词
D O I
10.1021/ac00021a017
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An immunoaffinity precolumn containing immobilized antibodies raised against the synthetic steroid hormone beta-19-nortestosterone, has been used for the automated sample pretreatment of urine samples containing beta-19-nortestosterone or the related steroids norethindrone and norgestrel. The sample pretreatment system was coupled on-line to a capillary GC. The on-line connection between the immunoaffinity precolumn and the capillary GC was realized with an interface that consisted of a 10 mm X 2 mm i.d. reversed-phase precolumn and a diphenyltetramethyldisilazane-deactivated GC retention gap. After preconcentration on the immunoaffinity precolumn the analytes were eluted and reconcentrated on the reversed-phase precolumn. Subsequently, this precolumn was desorbed with 75-mu-L of ethyl acetate, which was directly introduced into the retention gap by using partially concurrent solvent evaporation. The system allows the automated pretreatment and GC analysis of 5-25-mL urine samples for the ppt-level determination of 19-norsteroids. The general applicability and potential of on-line immunoaffinity-capillary GC systems are discussed.
引用
收藏
页码:2481 / 2487
页数:7
相关论文
共 20 条
  • [11] ANALYSIS OF PETROLEUM FRACTIONS BY ONLINE MICRO HPLC-HRGC COUPLING, INVOLVING INCREASED EFFICIENCY IN USING RETENTION GAPS BY PARTIALLY CONCURRENT SOLVENT EVAPORATION
    MUNARI, F
    TRISCIANI, A
    MAPELLI, G
    TRESTIANU, S
    GROB, K
    COLIN, JM
    [J]. JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1985, 8 (09): : 601 - 606
  • [12] EXTRACTION AND QUANTITATION OF CORTISOL BY USE OF HIGH-PERFORMANCE LIQUID AFFINITY-CHROMATOGRAPHY
    NILSSON, B
    [J]. JOURNAL OF CHROMATOGRAPHY, 1983, 276 (02): : 413 - 417
  • [13] ANALYSIS OF ALBUTEROL IN HUMAN-PLASMA BASED ON IMMUNOAFFINITY CHROMATOGRAPHIC CLEANUP COMBINED WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORIMETRIC DETECTION
    ONG, H
    ADAM, A
    PERREAULT, S
    MARLEAU, S
    BELLEMARE, M
    DUSOUICH, P
    BEAULIEU, N
    [J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 497 : 213 - 221
  • [14] REH E, 1989, J CHROMATOGR, V433, P119
  • [15] RAPID DUAL-COLUMN CHROMATOGRAPHIC ASSAY FOR RECOMBINANT LEUKOCYTE INTERFERON-ALPHA-2
    RYBACEK, L
    DANDREA, M
    TARNOWSKI, SJ
    [J]. JOURNAL OF CHROMATOGRAPHY, 1987, 397 : 355 - 364
  • [16] APPLICATION OF AN IMMUNOAFFINITY COLUMN SAMPLE CLEANUP TO THE DETERMINATION OF AFLATOXIN-M1 IN CHEESE
    SHARMAN, M
    PATEY, AL
    GILBERT, J
    [J]. JOURNAL OF CHROMATOGRAPHY, 1989, 474 (02): : 457 - 461
  • [17] IMMUNOAFFINITY PURIFICATION OF INDOLE-3-ACETAMIDE USING MONOCLONAL-ANTIBODIES
    ULVSKOV, P
    MARCUSSEN, J
    RAJAGOPAL, R
    PRINSEN, E
    RUDELSHEIM, P
    VANONCKELEN, H
    [J]. PLANT AND CELL PHYSIOLOGY, 1987, 28 (05) : 937 - 945
  • [18] MONOCLONAL ANTIBODY-MEDIATED CLEANUP PROCEDURE FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF CHLORAMPHENICOL IN MILK AND EGGS
    VANDEWATER, C
    TEBBAL, D
    HAAGSMA, N
    [J]. JOURNAL OF CHROMATOGRAPHY, 1989, 478 (01): : 205 - 215
  • [19] ASSAY FOR TRENBOLONE AND ITS METABOLITE 17-ALPHA-TRENBOLONE IN BOVINE URINE BASED ON IMMUNOAFFINITY CHROMATOGRAPHIC CLEANUP AND OFF-LINE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THIN-LAYER CHROMATOGRAPHY
    VANGINKEL, LA
    VANBLITTERSWIJK, H
    ZOONTJES, PW
    VANDENBOSCH, D
    STEPHANY, RW
    [J]. JOURNAL OF CHROMATOGRAPHY, 1988, 445 (02): : 385 - 392
  • [20] MULTI-IMMUNOAFFINITY CHROMATOGRAPHY - A SIMPLE AND HIGHLY SELECTIVE CLEANUP METHOD FOR MULTI-ANABOLIC RESIDUE ANALYSIS OF MEAT
    VANGINKEL, LA
    STEPHANY, RW
    VANROSSUM, HJ
    STEINBUCH, HM
    ZOMER, G
    VANDEHEEFT, E
    DEJONG, APJM
    [J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 489 (01): : 111 - 120