CHARACTERIZATION OF A DNA-BINDING PHOSPHOPROTEIN FROM VACCINIA VIRUS-REPLICATION COMPLEX

被引：23

作者：

NOWAKOWSKI, M

BAUER, W

KATES, J

机构：

[1] Department of Microbiology, Health Sciences Center, State University of New York, Stony Brook

来源：

VIROLOGY | 1978年 / 86卷 / 01期

关键词：

D O I：

10.1016/0042-6822(78)90022-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The DNA-protein complex which forms in the cytoplasm of HeLa cells during the course of vaccinia virus infection contains a single DNA-binding phosphoprotein, termed FP11. This phosphoprotein has been purified to near homogeneity by chromatography on denatured DNA-cellulose. Phosphoprotein FP11 has an apparent molecular weight of 34,000, as determined by electrophoresis in polyacrylamide gels containing sodium dodecyl sulfate. High-voltage paper electrophoresis following acid hydrolysis reveals that the polypeptide contains phosphothreonine but not phosphoserine. The in vivo phosphorylation occurs at a minimum of four distinct phosphothreonine residues. FP11 is an acidic protein with an isoelectric point lying in the pH region 5.2-5.5. © 1978.

引用

页码：217 / 225

页数：9