MYOSIN SYNTHESIS IN EMBRYONIC CHICKEN FIBROBLASTS

被引：15

作者：

YOUNG, RB

BERGEN, WG

BLAUWIEKEL, PB

机构：

[1] MICHIGAN STATE UNIV, DEPT BIOMECH, E LANSING, MI 48824 USA

[2] MICHIGAN STATE UNIV, DEPT ANIM HUSB, E LANSING, MI 48824 USA

来源：

JOURNAL OF CELL BIOLOGY | 1979年 / 81卷 / 01期

关键词：

D O I：

10.1083/jcb.81.1.115

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The rate of constitutive myosin synthesis was measured in cultures of replicating embryonic chicken skin fibroblasts by pulse labeling with [3H]leucine. These cells synthesized the 200,000-dalton heavy chain of myosin (MHC) at a rate of 3.2x103 molecules/cell/min. Additionally, an independent estimate of the MHC synthesis rate needed to maintain a constant level of constitutive MHC/cell was calculated from total protein content, percentage MHC, fibroblast doubling time, and MHC half-life. This calculated rate of ~2.9x103 molecules/cell/min was in close agreement with the measured rate. By comparison, the synthesis rate of myofibrillar MHC in fully activated muscle cell cultures was ~2.9x104 molecules/nucleus/min.

引用

页码：115 / 122

页数：8

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