HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CREATININE IN SERUM, AND A CORRELATION OF THE RESULTS WITH THOSE OF THE JAFFE AND ENZYMATIC METHODS

A high-performance liquid chromatographic method for the determination of creatinine in control sera is reported, based on its separation from deproteinated serum components on the ion-exchange material HEMA Bio 1000 SB and ultraviolet detection at 230 nm. Groups of eleven to fourteen samples of human serum and several control materials were simultaneously analysed by the Jaffe, enzymic ultraviolet and enzymic peroxidase aminophenazone methods. Another group (52-115 sera) was analysed for correlations with spectrophotometric methods. The precision of the chromatographic method ranges between 2.0 and 1.0% (relative standard deviation) for serum creatinine concentrations of 115.1 to 471 mumol/l, respectively. A very good accuracy was found in analyses of reference materials Kontrollogen-L and -LP. Some results of analyses of the other control sera were higher and the other lower than those obtained by the Jaffe and enzymic methods, because both interferences and enzyme inhibitors were encountered. Correlations between the chromatographic and spectrophotometric methods were good.