NONCOMPETITIVE IMMUNOASSAY OF THYROXINE USING A LIQUID-PHASE BINDING ASSAY

被引：16

作者：

HARA, T ^{[1
]}

NAKAMURA, K ^{[1
]}

SATOMURA, S ^{[1
]}

MATSUURA, S ^{[1
]}

机构：

[1] WAKO PURE CHEM IND LTD,OSAKA RES LABS,AMAGASAKI,HYOGO 661,JAPAN

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 03期

关键词：

D O I：

10.1021/ac00075a007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A thyroxine immunoassay was developed based on the principle of a liquid-phase binding assay. Thyroxine and peroxidase-(POD-) labeled anti-thyroxine monoclonal antibody (Fab'-POD) solutions ape mixed, incubated, and analyzed directly by high-performance liquid chromatography using a cation-exchange column and a postcolumn enzyme reaction apparatus. This system Separates antigen-antibody immune complex from free labeled antibody. The amount of labeled antibody is determined fluorophotometrically by assay of POD enzymatic activity. The amount of thyroxine-was determined from the chromatographic peak height of the immune complex. All thyroxine molecules formed immune complexes upon addition of excess Fab'-POD. A linear thyroxine dose-response curve was obtained because thyroxine molecules bound peroxidase-antibody conjugates in a 1:1 ratio. This simple, rapid, and convenient immunoassay method does not require an analyte derivative and can be used to measure other low molecular weight antigens.

引用

页码：351 / 354

页数：4

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