ECHINOMYCIN BINDING TO THE SEQUENCE CG(AT)NCG ALTERS THE STRUCTURE OF THE CENTRAL AT REGION

被引：18

作者：

FOX, KR

KENTEBE, E

机构：

[1] Department of Physiology and Pharmacology, University of Southampton, Southampton SO9 3TU, Bassett Crescent East

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 08期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/18.8.1957

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA fragments containing the sequence CG(AT)nCG have been used in footprinting experiments to assess the effect of echinomycin, which binds to the CG steps, on the structure of the central AT region. DNAase I normally cuts ApT much better than TpA; in the presence of the drug this preference is retained but cleavage at TpA is enhanced. Changes in cleavage by micrococcal nuclease have also been observed. Echinomycin renders alternate adenines hyperreactive to diethylpyrocarbonate. The results suggest that echinomycin induces structural changes in regions surrounding its binding site and that these can be cooperatively propagated over several turns of the DNA helix. © 1990 Oxford University Press.

引用

页码：1957 / 1963

页数：7