IN-VITRO RELEASE OF LECTINS FROM PHALLUSIA-MAMILLATA HEMOCYTES AFTER THEIR FRACTIONATION ON A DENSITY GRADIENT

被引：17

作者：

CAMMARATA, M ^{[1
]}

PARRINELLO, N ^{[1
]}

ARIZZA, V ^{[1
]}

机构：

[1] UNIV PALERMO,INST ZOOL,VIA ARCHIRAFI 18,I-90123 PALERMO,ITALY

来源：

JOURNAL OF EXPERIMENTAL ZOOLOGY | 1993年 / 266卷 / 04期

关键词：

D O I：

10.1002/jez.1402660410

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Hemocytes were fractionated by centrifugation on a discontinuous Percoll density gradient from the hemolymph of Phallusia mamillata. Results obtained from microcultures of the fractionated hemocytes, sugar-inhibition experiments, SDS-PAGE, and immunoblotting indicate that ''compartment cells'' release cellular-type (CL) lectins that are specific for alpha-lactose and lactulose. The released lectins have the same properties as the CL lectins that were previously isolated from sonicated unfractionated hemocytes, but they differ in terms of some molecular and immunological properties from the lectins (SL) purified from the serum. SLs were never found in the supernatants from microcultures of the fractionated hemocytes. (C) 1993 Wiley-Liss, Inc.

引用

页码：319 / 327

页数：9