A G-PROTEIN-COUPLED 130-KDA PHOSPHOLIPASE-C ISOZYME, PLC-BETA-4, FROM THE PARTICULATE FRACTION OF BOVINE CEREBELLUM

被引：14

作者：

MIN, DS ^{[1
]}

KIM, Y ^{[1
]}

LEE, YH ^{[1
]}

SUH, PG ^{[1
]}

RYU, SH ^{[1
]}

机构：

[1] POHANG UNIV SCI & TECHNOL, DEPT LIFE SCI, POB 125, POHANG 790600, SOUTH KOREA

来源：

FEBS LETTERS | 1993年 / 331卷 / 1-2期

关键词：

PHOSPHOLIPASE-C ISOZYME; BOVINE CEREBELLUM; G-PROTEIN-DEPENDENT ACTIVATION;

D O I：

10.1016/0014-5793(93)80293-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 130 kDa PLC isozyme was purified from the particulate fraction of bovine cerebellum. This PLC was recognized by a polyclonal antiserum generated against the purified 97 kDa PLC-beta4. Reconstitution of the purified 130 kDa PLC with the membranes of C6 Bu-1 cells in the presence of GTPgammaS or AlF4- resulted in PLC activation as well as the association of PLC with the membranes. Both the association and activation were revoked when the membrane was washed with 2 M KCl. The 97 kDa PLC-beta4 did not associate with membranes. These data suggest that the 130 kDa PLC is the intact form of PLC-beta4 the activity of which is likely to be regulated by a G-protein on the membrane.

引用

页码：38 / 42

页数：5

共 30 条

[1] INOSITOL PHOSPHATES AND CELL SIGNALING [J].

BERRIDGE, MJ ;

IRVINE, RF .

NATURE, 1989, 341 (6239) :197-205

[2]

BLANK JL, 1991, J BIOL CHEM, V266, P18206

[3]

BLANK JL, 1992, J BIOL CHEM, V267, P23069

[4] ISOLATION OF A PUTATIVE PHOSPHOLIPASE-C GENE OF DROSOPHILA, NORPA, AND ITS ROLE IN PHOTOTRANSDUCTION [J].

BLOOMQUIST, BT ;

SHORTRIDGE, RD ;

SCHNEUWLY, S ;

PERDEW, M ;

MONTELL, C ;

STELLER, H ;

RUBIN, G ;

PAK, WL .

CELL, 1988, 54 (05) :723-733

[5] ISOZYME-SELECTIVE STIMULATION OF PHOSPHOLIPASE C-BETA-2 BY G-PROTEIN BETA-GAMMA-SUBUNITS [J].

CAMPS, M ;

CAROZZI, A ;

SCHNABEL, P ;

SCHEER, A ;

PARKER, PJ ;

GIERSCHIK, P .

NATURE, 1992, 360 (6405) :684-686

[6] IDENTIFICATION, PURIFICATION AND CHARACTERIZATION OF A NOVEL PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C, A 3RD MEMBER OF THE BETA-SUBFAMILY [J].

CAROZZI, AJ ;

KRIZ, RW ;

WEBSTER, C ;

PARKER, PJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 210 (02) :521-529

[7]

JHON DY, 1993, J BIOL CHEM, V268, P6654

[8] DETERMINATION OF THE PRIMARY STRUCTURE OF PLC-154 DEMONSTRATES DIVERSITY OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C ACTIVITIES [J].

KATAN, M ;

KRIZ, RW ;

TOTTY, N ;

PHILP, R ;

MELDRUM, E ;

ALDAPE, RA ;

KNOPF, JL ;

PARKER, PJ .

CELL, 1988, 54 (02) :171-177

[9] SUBUNITS-BETA-GAMMA OF HETEROTRIMERIC G-PROTEIN ACTIVATE BETA-2 ISOFORM OF PHOSPHOLIPASE-C [J].

KATZ, A ;

WU, DQ ;

SIMON, MI .

NATURE, 1992, 360 (6405) :686-689

[10]

KRIZ R, 1990, CIBA F SYMP, V150, P112

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