MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF ENZYMATICALLY SYNTHESIZED RNA UP TO 150 KDA

被引：93

作者：

KIRPEKAR, F

NORDHOFF, E

KRISTIANSEN, K

ROEPSTORFF, P

LEZIUS, A

HAHNER, S

KARAS, M

HILLENKAMP, F

机构：

[1] UNIV MUNSTER,INST MED PHYS & BIOPHYS,D-48149 MUNSTER,GERMANY

[2] UNIV MUNSTER,INST BIOCHEM,D-48149 MUNSTER,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 19期

关键词：

D O I：

10.1093/nar/22.19.3866

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

引用

页码：3866 / 3870

页数：5

共 21 条

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