MICELLAR-ENHANCED SYNCHRONOUS-DERIVATIVE FLUORESCENCE DETERMINATION OF DERIVATIZED FOLIC-ACID IN PHARMACEUTICAL PREPARATIONS

被引：17

作者：

BLANCO, CC

CARRETERO, AS

GUTIERREZ, AF

CEBA, MR

机构：

[1] Department of Analytical Chemistry, Faculty of Sciences, University of Granada

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1995年 / 13卷 / 08期

关键词：

FLUORESCAMINE; FOLIC ACID; MICELLAR MEDIA; PHARMACEUTICAL ANALYSIS; SYNCHRONOUS-DERIVATIVE SPECTROFLUOROMETRY;

D O I：

10.1016/0731-7085(95)01530-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and inexpensive micellar-enhanced synchronous-derivative spectrofluorimetric method is described for the determination of folic acid in pharmaceutical preparations. The method is based upon derivatization of the vitamin with fluorescamine in acid solution and enhancement of the fluorescence with a surfactant. Linear fluorimetric analytical curves were obtained for folic acid concentrations of 0.05-6 mu g ml(-1) (detection limit 0.012 mu g ml(-1)); the RSD for 1 mu g ml(-1) of folic acid was 2.82%. The method has been applied to the determination of folic acid synthetic mixtures and pharmaceutical preparations.

引用

页码：1019 / 1025

页数：7

共 37 条

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