LIQUID COLUMN CHROMATOGRAPHY OF NUCLEOSIDE MONO-PHOSPHATES ON DEAE-SPHERON 1000

The ion exchange capacity, radiation stability and particle distribution have been estimated for two fractions of the weak anion exchanger DEAE-Spheron 1000. Capacity factors are reported for eight nucleotides measured under various conditions in citrate and formate buffers. This type of sorbent may be used for the separation of deoxy- or ribonucleotides under mild conditons in ammonium formate buffer. No interferences from other substances which are usually found in samples of biological origin have been observed. © 1979.