HOMOLOGOUS RECOMBINATION IN BOVINE PAPILLOMAVIRUS SHUTTLEVECTER - EFFECT OF RELATIVE ORIENTATION OF SUBSTRATE SEQUENCES

被引：2

作者：

NAITO, A

KITAMURA, Y

YOSHIKURA, H

机构：

[1] Department of Bacteriology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, 113, 7-3-1, Hongo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90521-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Relative orientation of recombination substrates, neo gene, strongly influenced homologous recombination events in a bovine papillomavirus shuttle vector. Between direct repeats, recombination occurred at a high frequency while between inverted repeats, it was rare. Double strand break near the mutation site increased the recombination frequency between inverted repeats but not between direct repeats. Formation of long heteroduplex as a recombination intermediate may explain this apparently paradoxical phenomenon. © 1991.

引用

页码：305 / 312

页数：8