SELECTIVITY IN THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF ECDYSTEROIDS

被引:16
作者
LAFONT, R [1 ]
KAOUADJI, N [1 ]
MORGAN, ED [1 ]
WILSON, ID [1 ]
机构
[1] UNIV KEELE, DEPT CHEM, KEELE ST5 5BG, STAFFS, ENGLAND
关键词
D O I
10.1016/0021-9673(94)85206-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Phytoecdysteroids are a large family of plant compounds structurally related to ecdysone, and the complete HPLC separation of all these compounds requires the development of various complementary chromatographic systems. This paper describes a set of reversed-phase (RP),and normal-phase (NP) HPLC systems that can be used to separate ecdysteroids efficiently. A suitable combination of these NP- and RP-HPLC systems may allow the complete resolution of complex mixtures, but clearly the use of only two systems is not sufficient. Several original solvent systems are described and some examples are given to illustrate their selectivity towards the most common modifications of the ecdysteroid molecule.
引用
收藏
页码:55 / 67
页数:13
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