DIFFERENTIALLY EXPRESSED GENES AFTER PERIPHERAL-NERVE INJURY

被引:66
作者
GILLEN, C
GLEICHMANN, M
SPREYER, P
MULLER, HW
机构
[1] UNIV DUSSELDORF, DEPT NEUROL, MOLEC NEUROBIOL LAB, D-40225 DUSSELDORF, GERMANY
[2] UNIV DUSSELDORF, BIOMED RES CTR, D-40225 DUSSELDORF, GERMANY
关键词
SCIATIC NERVE; REGENERATION; DEGENERATION; DIFFERENTIAL SCREENING; CDNA LIBRARY;
D O I
10.1002/jnr.490420203
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In an attempt to identify genes associated with Wallerian degeneration and peripheral nerve regeneration we have performed differential hybridization screening of a cDNA library from crushed rat sciatic nerve (7 days postlesion) using radioactively labeled cDNA prepared from poly(A)(+) RNA of normal vs, crushed nerve, Screening of 5,000 randomly selected colonies yielded 24 distinct clones that were regulated following nerve injury, Fifteen of the differentially expressed sequences could be classified as induced, whereas 9 sequences appeared to be repressed at 1 week postcrush, Sequencing and computer-assisted sequence comparison revealed 3 classes of regulated cDNA clones representing 1) novel gene sequences (8 clones) including 3 transcripts containing a repetitive ''brain identifier'' (ID) element; 2) identified genes (7 clones) with previously undetected expression in the peripheral nervous system (PNS), such as apolipoprotein D, peripheral myelin protein 22kD (PMP22), SPARC (secreted protein, acidic and rich in cysteine), sulfated glycoprotein SGP-1, apoferritin, decorin, and X16/SRp20; and 3) identified genes (9 clones) with known expression in the PNS including, e,g,, the myelin protein P-0, gamma-actin, vimentin, alpha-tubulin, chargerin II, and cytochrome c-oxidase subunit I, Northern blot and polymerase chain reaction analyses with RNA from crushed and transected nerve demonstrated that sequences with related function, like the group of myelin genes, cytoskeleton genes, genes involved in RNA processing and translation, in lipid transport or energy metabolism showed closely related temporal patterns of expression during nerve degeneration and regeneration, Finally, we compared the differentially expressed genes identified at 7 days after crush injury (this investigation) with the regulated sequences isolated previously by De Leon et al, (J Neurosci Res 29:437-488, 1991) from a 3 day postcrush sciatic nerve cDNA library. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:159 / 171
页数:13
相关论文
共 99 条
[1]   A DIFFERENTIALLY EXPRESSED MURINE RNA ENCODING A PROTEIN WITH SIMILARITIES TO 2 TYPES OF NUCLEIC-ACID BINDING MOTIFS [J].
AYANE, M ;
PREUSS, U ;
KOHLER, G ;
NIELSEN, PJ .
NUCLEIC ACIDS RESEARCH, 1991, 19 (06) :1273-1278
[2]   MACROPHAGE-MEDIATED MYELIN-RELATED MITOGENIC FACTOR FOR CULTURED SCHWANN-CELLS [J].
BAICHWAL, RR ;
BIGBEE, JW ;
DEVRIES, GH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (05) :1701-1705
[3]   IDENTIFICATION OF DIFFERENTIALLY EXPRESSED MESSENGER-RNA SPECIES BY AN IMPROVED DISPLAY TECHNIQUE (DDRT-PCR) [J].
BAUER, D ;
MULLER, H ;
REICH, J ;
RIEDEL, H ;
AHRENKIEL, V ;
WARTHOE, P ;
STRAUSS, M .
NUCLEIC ACIDS RESEARCH, 1993, 21 (18) :4272-4280
[4]  
BEUCHE W, 1984, J NEUROCYTOL, V13, P767, DOI 10.1007/BF01148493
[5]   ANALYSIS OF THE RNA-RECOGNITION MOTIF AND RS AND RGG DOMAINS - CONSERVATION IN METAZOAN PRE-MESSENGER-RNA SPLICING FACTORS [J].
BIRNEY, E ;
KUMAR, S ;
KRAINER, AR .
NUCLEIC ACIDS RESEARCH, 1993, 21 (25) :5803-5816
[6]  
BOSCH EP, 1989, J NEUROSCI, V9, P3690
[7]   DIFFERENTIAL EXPRESSION OF 2 MESSENGER-RNA SPECIES INDICATES A DUAL FUNCTION OF PERIPHERAL MYELIN PROTEIN PMP22 IN CELL-GROWTH AND MYELINATION [J].
BOSSE, F ;
ZOIDL, G ;
WILMS, S ;
GILLEN, CP ;
KUHN, HG ;
MULLER, HW .
JOURNAL OF NEUROSCIENCE RESEARCH, 1994, 37 (04) :529-537
[8]   THE PRIMARY STRUCTURE OF THE ALPHA-SUBUNIT OF HUMAN ELONGATION FACTOR-I - STRUCTURAL ASPECTS OF GUANINE-NUCLEOTIDE-BINDING SITES [J].
BRANDS, JHGM ;
MAASSEN, JA ;
VANHEMERT, FJ ;
AMONS, R ;
MOLLER, W .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 155 (01) :167-171
[9]   A CDNA SEQUENCE ENCODING CYTOSKELETAL GAMMA-ACTIN FROM RAT [J].
BROWN, CW ;
MCHUGH, KM ;
LESSARD, JL .
NUCLEIC ACIDS RESEARCH, 1990, 18 (17) :5312-5312
[10]   DIFFERENTIAL EXPRESSION OF VIMENTIN IN RAT PROSTATIC TUMORS [J].
BUSSEMAKERS, MJG ;
VERHAEGH, GWCT ;
VANBOKHOVEN, A ;
DEBRUYNE, FMJ ;
SCHALKEN, JA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 182 (03) :1254-1259