CHARACTERIZATION OF A LYSINE-TO-GLUTAMIC ACID MUTATION IN A CONSERVATIVE SEQUENCE OF FARNESYL DIPHOSPHATE SYNTHASE FROM SACCHAROMYCES-CEREVISIAE

被引：68

作者：

BLANCHARD, L

KARST, F

机构：

[1] Laboratoire de Biochimie et Génétique des Microorganismes, Institut de Biologie Moléculaire et d'Ingéniérie Génétique, Université de Poitiers

来源：

GENE | 1993年 / 125卷 / 02期

关键词：

D O I：

10.1016/0378-1119(93)90326-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mutant gene erg20-2 was isolated from a yeast strain defective in farnesyl diphosphate synthase (FPPS). This strain had the unusual property of excreting prenyl alcohols such as geraniol. The nucleotide (nt) sequence, compared with that of the wild-type gene, showed a single nt change, resulting in a Lys197 --> Glu substitution in FPPS which is directly involved in terpenic alcohol formation. In addition, disruption of ERG20 revealed that in yeast no other prenyl transferase is able to synthesize the FPP molecules required for essential non-sterol metabolites.

引用

页码：185 / 189

页数：5

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