ANTIBACTERIAL ANTIBODIES IN EPSTEIN-BARR-VIRUS (EBV)-TRANSFORMED OLIGOCLONAL B-CELL LINES ESTABLISHED FROM NORMAL PERSONS AND AUTOIMMUNE-DISEASE PATIENTS

被引：1

作者：

NAGATSUKA, Y

HANAZAWA, S

KOROIWA, Y

FUKUDA, T

SUGANUMA, T

ONO, Y

机构：

[1] Department of Microbiology, Nihon University, School of Medicine, Tokyo

[2] Mitsui Pharmaceutical Co., Ltd

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 04期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00944.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have established 950 and 430 oligoclonal B-lymphoblastoid cell lines (LCL) from two normal persons and eight autoimmune disease patients, respectively by using Epstein-Barr virus (EBV)-induced transformation. To re-evaluate the EBV technique for production of human monoclonal antibodies (mAb) related to infectious disease, we screened these oligoclonal LCLs for antibodies against 31 bacterial strains systematically. A total of 74 cultures out of 1380 were reactive to a total of 18 strains out of 31. Among these, eight cultures showed 10(-3) antibody (Ab) titers to Pseudomonas aeruginosa serotypes C, E, F and I, Staphylococcus aureus, Serratia marcescens and Bacillus cereus. Ten cultures showed 10(-2) Ab titers to Ps. aeruginosa serotypes D, E, F and I, Ps. maltophilia, Staph. epidermidis, Klebsiella ozaenae, Ser. marcescens and B. subtilis. The results reveal the further possibilities for the EBV technique to produce various infectious disease-related human mAbs.

引用

页码：206 / 209

页数：4

共 9 条

[1] EPSTEIN-BARR VIRUS SUSCEPTIBILITY OF NORMAL HUMAN B-LYMPHOCYTE POPULATIONS [J].