GALACTOSYLATION OF CYCLODEXTRINS AND BRANCHED CYCLODEXTRINS BY ALPHA-GALACTOSIDASES

被引：36

作者：

HARA, K

FUJITA, K

KUWAHARA, N

TANIMOTO, T

HASHIMOTO, H

KOIZUMI, K

KITAHATA, S

机构：

[1] ENSUIKO SUGAR REFINING CO LTD, CARBOHYDRATE RES LAB, TSURUMI KU, YOKOHAMA 230, KANAGAWA, JAPAN

[2] MUKOGAWA WOMENS UNIV, FAC PHARMACEUT SCI, NISHINOMIYA, HYOGO 663, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 04期

关键词：

D O I：

10.1271/bbb.58.652

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgalactosylated derivatives of cyclodextrins (CDs) and glucosyl and maltosyl CDs (G1- and G2-CDs) were synthesized by alpha-galactosidases from coffee bean and Mortierella vinacea (M. vinacea). The structures of the transfer products were analyzed by FAB-mass, C-13-NMR and methylation. Coffee bean alpha-galactosidase transferred a galactosyl residue not only to side chains of G1-CDs and G2-CDs, but also directly to CD rings. M. vinacea alpha-galactosidase transferred a galactosyl residue only to side chains of G2-CDs.

引用

页码：652 / 659

页数：8

共 6 条

[1] ASSIGNMENT OF PROTON-DECOUPLED C-13 SPECTRA OF COMPLEX-MOLECULES BY USING POLARIZATION TRANSFER SPECTROSCOPY - A SUPERIOR METHOD TO OFF-RESONANCE DECOUPLING [J].