PREPARATION, ISOLATION, AND CHARACTERIZATION OF NOVEL HETEROGENEOUS BRANCHED CYCLOMALTOOLIGOSACCHARIDES HAVING BETA-D-GALACTOSYL RESIDUE(S) ON THE SIDE-CHAIN

被引：30

作者：

KOIZUMI, K

TANIMOTO, T

FUJITA, K

HARA, K

KUWAHARA, N

KITAHATA, S

机构：

[1] ENSUIKO SUGAR REFINING CO LTD,CARBOHYDRATE RES LAB,TSURUMI KU,YOKOHAMA 230,JAPAN

[2] OSAKA MUNICIPAL TECH RES INST,JYOTO KU,OSAKA 536,JAPAN

来源：

CARBOHYDRATE RESEARCH | 1993年 / 238卷

关键词：

D O I：

10.1016/0008-6215(93)87006-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgalactosylated products of branched cyclodextrins (glucosyl-alphaCD, -betaCD, -gammaCD, and maltosyl-alphaCD, -betaCD, -gammaCD)were synthesized by beta-D-galactosidases from Bacillus circulans and Penicillium multicolor using lactose as a donor substrate and branched CDs as acceptors. Eighteen beta-D-galactosylated branched CDs were isolated and purified by HPLC. Their structures were elucidated by FABMS and C-13 NMR spectroscopies, and methylation analysis. The chromatographic behavior of these novel heterogeneous branched CDs on three HPLC columns of different separation modes was compared.

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页码：75 / 91

页数：17

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