ESTIMATION OF THE DISTRIBUTIONS OF CHAIN-LENGTH OF AMYLOPECTINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION

被引：131

作者：

KOIZUMI, K ^{[1
]}

FUKUDA, M ^{[1
]}

HIZUKURI, S ^{[1
]}

机构：

[1] KAGOSHIMA UNIV,DEPT BIOCHEM SCI & TECHNOL,KAGOSHIMA 890,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 585卷 / 02期

关键词：

D O I：

10.1016/0021-9673(91)85082-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

High-performance anion-exchange chromatography for a detailed estimation of the distribution of chain length of amylopectins was developed using a pulsed amperometric detector under alkaline conditions. As maltosaccharides having different degrees of polymerization, which were produced by debranching of amylopectin with isoamylase, exhibit different pulsed amperometric detector responses, the individual maltosaccharides (degree of polymerization 6-17) were isolated by high-performance liquid chromatography on an amino column and an octadecylsilane column to use as quantification standards. By the use of this high-performance anion-exchange chromatography the chain length distributions of some typical amylopectins were characterized in detail.

引用

页码：233 / 238

页数：6

共 11 条

[1] RELATIONSHIP BETWEEN THE DISTRIBUTION OF THE CHAIN-LENGTH OF AMYLOPECTIN AND THE CRYSTALLINE-STRUCTURE OF STARCH GRANULES [J].

HIZUKURI, S .

CARBOHYDRATE RESEARCH, 1985, 141 (02) :295-306

[2] POLYMODAL DISTRIBUTION OF THE CHAIN LENGTHS OF AMYLOPECTINS, AND ITS SIGNIFICANCE [J].

HIZUKURI, S .

CARBOHYDRATE RESEARCH, 1986, 147 (02) :342-347

[3] FINE-STRUCTURE OF WHEAT AMYLOPECTIN - THE MODE OF A-CHAIN TO B-CHAIN BINDING [J].

HIZUKURI, S ;

MAEHARA, Y .

CARBOHYDRATE RESEARCH, 1990, 206 (01) :145-159

[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF GLUCO-OLIGOMERS ON C-18-BONDED VINYL ALCOHOL COPOLYMER GEL WITH ALKALINE ELUENTS [J].

KOIZUMI, K ;

UTAMURA, T .

JOURNAL OF CHROMATOGRAPHY, 1988, 436 (02) :328-333

[5]

KOIZUMI K, 1989, J CHROMATOGR, V464, P365

[6] ANALYSES OF HOMOGENEOUS D-GLUCO-OLIGOSACCHARIDES AND D-GLUCO-POLYSACCHARIDES (DEGREE OF POLYMERIZATION UP TO ABOUT 35) BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND THIN-LAYER CHROMATOGRAPHY [J].

KOIZUMI, K ;

UTAMURA, T ;

OKADA, Y .

JOURNAL OF CHROMATOGRAPHY, 1985, 321 (01) :145-157

[7] STARCH PHOSPHATE .2. ISOLATION OF GLUCOSE 3-PHOSPHATE AND MALTOSE PHOSPHATE BY ACID HYDROLYSIS OF POTATO STARCH [J].

TABATA, S ;

HIZUKURI, S .

STARKE, 1971, 23 (08) :267-&

[8] STUDIES ON STARCH PHOSPHATE .6. LOCATION OF PHOSPHATE GROUPS IN POTATO AMYLOPECTIN [J].

TAKEDA, Y ;

HIZUKURI, S .

CARBOHYDRATE RESEARCH, 1982, 102 (APR) :321-327

[9] STRUCTURES OF RICE AMYLOPECTINS WITH LOW AND HIGH AFFINITIES FOR IODINE [J].

TAKEDA, Y ;

HIZUKURI, S ;

JULIANO, BO .

CARBOHYDRATE RESEARCH, 1987, 168 (01) :79-88

[10] PHYSICOCHEMICAL PROPERTIES OF SWEET-POTATO STARCHES [J].

TAKEDA, Y ;

TOKUNAGA, N ;

TAKEDA, C ;

HIZUKURI, S .

STARCH-STARKE, 1986, 38 (10) :345-350

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