OPSONIN-INDEPENDENT ADHERENCE AND INTRACELLULAR DEVELOPMENT OF LEGIONELLA-PNEUMOPHILA WITHIN U-937 CELLS

被引:36
作者
RODGERS, FG
GIBSON, FC
机构
[1] Department of Microbiology, Spaulding Life Science Center, University of New Hampshire, Durham
关键词
LEGIONELLA; U-937; CELLS; INTRACELLULAR REPLICATION; OPSONIN-INDEPENDENT UPTAKE;
D O I
10.1139/m93-103
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The infectious process was evaluated by viable bacterial cell colony counts and documented by transmission and scanning electron microscopy. In the absence of opsonins, wash-resistant bacterial adherence to host cells occurred within 1 h and attachment of 1 or 2 organisms per U-937 host cell involved close surface interactions at the prokaryotic and eukaryotic membranes. Intracellular multiplication of bacteria was maximal by 24 h after inoculation of cell monolayers. Release of L. pneumophila from these cells appeared as a lytic process that resulted in an increase in the numbers of microorganisms in the extracellular fluids and a concomitant decline in the number of intracellular bacteria. The course of cellular infection was completed by 72 h. The cellular and ultrastructural events of L. pneumophila adherence and uptake by U-937 cells in the absence of antibody or complement have been defined. In addition, this work further establishes the U-937 cell as a suitable model for investigating Legionella,- host cell interactions.
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页码:718 / 722
页数:5
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