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CHARACTERIZATION OF OSTERTAGIA-OSTERTAGI ANTIGENS BY THE DIFFERENT BOVINE IMMUNOGLOBULIN ISOTYPES

被引：18
作者：
HILDERSON, H
DE GRAAF, DC
VERCRUYSSE, J
BERGHEN, P
机构：
[1] Department of Parasitology, Faculty of Veterinary Medicine, University of Ghent, B-9000 Ghent
来源：
RESEARCH IN VETERINARY SCIENCE | 1993年 / 55卷 / 02期
关键词：
D O I：
10.1016/0034-5288(93)90082-Q
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Antigenic differences between the developmental stages of Ostertagia ostertagi were studied by SDS-PAGE and Western blotting. Gel electrophoresis showed a complex protein pattern different for every stage with the O ostertagi fourth stage larvae (L4) showing an intermediate protein pattern between the third stage larvae (L3) and the adult stage. Immunoblotting showed that IgG1, IgG2 and IgM immunoglobulins present in serum from uninfected calves identified several O ostertagi antigens at every stage. When using serum from O ostertagi infected calves, O ostertagi specific IgG1 was the predominant bovine immunoglobulin. Specific IgG2 and IgM responses were also observed, while specific IgA antibodies were hardly detectable. Severe IgG1 cross reactivity was demonstrated when using anti-Cooperia oncophora serum.
引用
收藏
页码：203 / 208
页数：6
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