PURIFICATION AND CHARACTERIZATION OF THYROID TRANSCRIPTION FACTOR-2

被引：43

作者：

CIVITAREALE, D ^{[1
]}

SAIARDI, A ^{[1
]}

FALASCA, P ^{[1
]}

机构：

[1] CNR,IST MED SPERIMENTALE,I-00100 ROME,ITALY

来源：

BIOCHEMICAL JOURNAL | 1994年 / 304卷

关键词：

D O I：

10.1042/bj3040981

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thyroid transcription factor 2 binds to the promoters of both thyroglobulin and thyroperoxidase genes, two markers of thyroid tissue differentiation, and its binding modulates the activity of both promoters. In this paper we describe the purification of thyroid transcription factor 2 essentially to homogeneity and demonstrate that it is a thyroid-specific DNA-binding protein. Furthermore, we provide a biochemical characterization suggesting that thyroid transcription factor 2 binds to DNA as a dimer and that it is a zinc-finger DNA-binding protein regulated in vitro by the redox state.

引用

页码：981 / 985

页数：5

共 27 条

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