A NOVEL TECHNIQUE FOR THE PREPARATION OF OSMOTICALLY STABILIZED AND PERMEABILIZED CELLS OF EXTREMELY HALOPHILIC BACTERIA

被引：10

作者：

DSOUZA, SE ^{[1
]}

ALTEKAR, W ^{[1
]}

DSOUZA, SF ^{[1
]}

机构：

[1] BHABHA ATOM RES CTR,DIV FOOD TECHNOL & ENZYME ENGN,BOMBAY 400085,INDIA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1992年 / 24卷 / 3-4期

关键词：

HALOBACTERIUM; CROSS-LINKING; OSMOTIC STABILIZATION; CELL PERMEABILIZATION;

D O I：

10.1016/0165-022X(94)90076-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The cells of Haloferax mediterannei were stabilized by cross-linking with 0.5% glutaraldehyde for 10 min. Such cells were found to be osmotically stable even when suspended in water. The stabilized cells could be permeabilized by treatment with chloroform without leakage of intracellular components. No significant difference in the properties of an intracellular enzyme aldolase was observed, using either cell-free extract or the osmotically stabilized and permeabilized cells. This novel technique can serve as a useful tool for studying in situ regulatory characteristics of intracellular functions in halobacteria and can also help in their re-use under more stabilized conditions for biotechnological applications.

引用

页码：239 / 247

页数：9

共 31 条

[21]

MATLIB M A, 1978, Archives of Biochemistry and Biophysics, V191, P426, DOI 10.1016/0003-9861(78)90380-6

[22]

MICK GJ, 1988, J BIOL CHEM, V263, P10667

[23] NOVEL-APPROACH TO THE STUDY OF THE REGULATION OF HORMONE-SENSITIVE LIPASE IN RAT ADIPOCYTES - PERMEABILIZATION OF CELLS WITH DIGITONIN [J].

MOONEY, RA ;

EBERSOHL, RD ;

MCDONALD, JM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 136 (03) :603-608

[24]

MOONEY RA, 1989, J BIOL CHEM, V264, P6850

[25]

RAWAL N, 1988, INDIAN J BIOCHEM BIO, V25, P674

[26]

Reichlin M, 1980, Methods Enzymol, V70, P159

[27]

RODRIGUEZVALERA F, 1980, J GEN MICROBIOL, V119, P535

[28]

SIBLEY JA, 1949, J BIOL CHEM, V117, P859

[29] COMPLEXES OF SEQUENTIAL METABOLIC ENZYMES [J].

SRERE, PA .

ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :89-124

[30] EFFECT OF CROSSLINKING ON MITOCHONDRIAL STRUCTURE AND FUNCTION [J].

TINBERG, HM ;

LEE, C .

JOURNAL OF SUPRAMOLECULAR STRUCTURE, 1975, 3 (03) :275-283

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