LUFFIN-S - A SMALL NOVEL RIBOSOME-INACTIVATING PROTEIN FROM LUFFA-CYLINDRICA - CHARACTERIZATION AND MECHANISM STUDIES

被引：43

作者：

GAO, WD ^{[1
]}

LING, J ^{[1
]}

ZHONG, XM ^{[1
]}

LIU, WY ^{[1
]}

ZHANG, RP ^{[1
]}

YANG, HL ^{[1
]}

CAO, HT ^{[1
]}

ZHANG, ZC ^{[1
]}

机构：

[1] ACAD SINICA,SHANGHAI INST BIOCHEM,SHANGHAI 200031,PEOPLES R CHINA

来源：

FEBS LETTERS | 1994年 / 347卷 / 2-3期

关键词：

RIBOSOME INACTIVATING PROTEIN; LUFFIN; RNA N-GLYCOSIDASE; PHOSPHODIESTERASE; IMMUNOTOXIN;

D O I：

10.1016/0014-5793(94)00554-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We purified and characterized a novel RIP (ribosome inactivating protein), Luffin-S from the seeds of Luffa cylindrica. Different from Luffin-A and B, which are RNA N-glycosidases with molecular weights of 27 and 28 kDa, respectively, Luffin-S has an M.W. of only approx. 10 kDa, much smaller than any other RIPs so far investigated. Its abundant resources, toxicity similar to TCS in a cell-free protein synthesis system and unique mechanism as phosphodiesterase, like alpha-sarcin, promisingly make it a potential toxic moiety of immunotoxin.

引用

页码：257 / 260

页数：4

共 14 条

[1] POLYPEPTIDE SYNTHESIS WITH RIBONUCLEASE-DIGESTED RIBOSOMES [J].

CAHN, F ;

SCHACHTE.EM ;

RICH, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 209 (02) :512-&

[2]

CHAN YL, 1983, J BIOL CHEM, V258, P2768

[3]

ENDO Y, 1987, J BIOL CHEM, V262, P8128

[4]

ENDO Y, 1982, J BIOL CHEM, V257, P9054

[5]

GLOSSMANN H, 1971, J BIOL CHEM, V246, P6339

[6] CONTROL OF GLOBIN SYNTHESIS - ROLE OF HEME [J].

HUNT, T ;

VANDERHOFF, G ;

LONDON, IM .

JOURNAL OF MOLECULAR BIOLOGY, 1972, 66 (03) :471-+

[7] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[8] TRICHOSANTHIN, A POTENT HIV-1 INHIBITOR, CAN CLEAVE SUPERCOILED DNA INVITRO [J].

LI, MX ;

YEUNG, HW ;

PAN, LP ;

CHAN, SI .

NUCLEIC ACIDS RESEARCH, 1991, 19 (22) :6309-6312

[9] GLQ223 - AN INHIBITOR OF HUMAN IMMUNODEFICIENCY VIRUS-REPLICATION IN ACUTELY AND CHRONICALLY INFECTED-CELLS OF LYMPHOCYTE AND MONONUCLEAR PHAGOCYTE LINEAGE [J].

MCGRATH, MS ;

HWANG, KM ;

CALDWELL, SE ;

GASTON, I ;

LUK, KC ;

WU, P ;

NG, VL ;

CROWE, S ;

DANIELS, J ;

MARSH, J ;

DEINHART, T ;

LEKAS, PV ;

VENNARI, JC ;

YEUNG, HW ;

LIFSON, JD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2844-2848

[10] ALPHA-SARCIN CLEAVES RIBOSOMAL-RNA AT THE ALPHA-SARCIN SITE IN THE ABSENCE OF RIBOSOMAL-PROTEINS [J].

MILLER, SP ;

BODLEY, JW .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 154 (01) :404-410

← 1 2 →