TRICHOSANTHIN, A POTENT HIV-1 INHIBITOR, CAN CLEAVE SUPERCOILED DNA INVITRO

被引：94

作者：

LI, MX

YEUNG, HW

PAN, LP

CHAN, SI

机构：

[1] CALTECH,ARTHUR AMOS NOYES LAB CHEM PHYS,127-72,PASADENA,CA 91125

[2] CHINESE UNIV HONG KONG,CHINESE MEDICINAL MAT RES CTR,SHA TIN,HONG KONG

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 22期

关键词：

D O I：

10.1093/nar/19.22.6309

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trichosanthin, an abortifacient, immunosuppressive and anti-tumor protein purified from the traditional Chinese herb medicine Tian Hua Fen, is a potent inhibitor against HIV-1 replication. Under normal enzymatic digestion conditions, trichosanthin cleaves the supercoiled double-stranded DNA to produce nicked circular and linear DNA. Trichosanthin has no effect on linear double-stranded DNA. Neither does it convert relaxed circular duplex DNA into a supercoiled form in the presence of ATP. Thus trichosanthin is not a DNA gyrase. However, trichosanthin can cleave the relaxed circular DNA into a linear form, indicating that both the circular as well as the supercoiled forms are essential for trichosanthin recognition. In addition, trichosanthin contains one calcium metal ion per protein molecule, which presumably is related to its endonucleolytic activity.

引用

页码：6309 / 6312

页数：4

共 27 条

[1] SIGN INVERSION MECHANISM FOR ENZYMATIC SUPERCOILING OF DNA [J].