SENSITIVE, INDIRECT PHOTOMETRIC DETECTOR FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING A LIGHT-EMITTING DIODE

被引：14

作者：

BERTHOD, A ^{[1
]}

GLICK, M ^{[1
]}

WINEFORDNER, JD ^{[1
]}

机构：

[1] UNIV FLORIDA,DEPT CHEM,GAINESVILLE,FL 32611

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 502卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)89595-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A low-noise detector for indirect photometric detection has been constructed using a highly stable source -a light-emitting diode (LED). Use of the detector is demonstrated for reversed-phase liquid chromatography by adding methylene blue to the mobile phase to make a background signal. The indirect determination of alcohols by their effect on methylene blue concentration distribution is demonstrated, and an investigation is made into the conditions for high sensitivity. Because the source exhibits low noise, the detection limits for alcohols are as low as more complex and expensive detection methods, despite the lower radiant power of the LED. Detection limits for nine alcohols are below μg injected amounts. © 1990.

引用

页码：305 / 315

页数：11

共 19 条

[11] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF LOW-MOLECULAR-WEIGHT ALIPHATIC-ALCOHOLS USING INDIRECT PHOTOMETRIC DETECTION [J].