WHEAT PLANTS REGENERATED FROM EMBRYO CELL-CULTURES

Wheat cultures capable of plant regeneration were obtained from young embryos of Triticum aestivum cv. Chinese Spring and cv. Salmon, and were maintained by subculturing them every 30 to 40 days on RM-64 basal medium supplemented with 2.0mg/l of 2, 4-D. Fourteen-day old embryos were at the optimum age for the induction of cultures capable of plant regeneration. Histological observations showed many shoot apexes on the surface of these cultures. The cultures remained capable of differentiation for about 8 months, and complete plants were restored from them. Cultures from wheat embryos may be useful in mutant selection studies. © 1979, The Genetics Society of Japan. All rights reserved.