HYALURONIC-ACID CAPSULE IS A VIRULENCE FACTOR FOR MUCOID GROUP-A STREPTOCOCCI

被引：291

作者：

WESSELS, MR ^{[1
]}

MOSES, AE ^{[1
]}

GOLDBERG, JB ^{[1
]}

DICESARE, TJ ^{[1
]}

机构：

[1] BETH ISRAEL HOSP, DIV INFECT DIS, BOSTON, MA 02115 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 19期

关键词：

BACTERIAL POLYSACCHARIDE; RHEUMATIC FEVER; PHAGOCYTOSIS; M-PROTEIN;

D O I：

10.1073/pnas.88.19.8317

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mucoid strains of group A Streptococcus have been associated with recent outbreaks of acute rheumatic fever. The mucoid colony morphology of these strains is a result of abundant production of capsular polysaccharide, which is composed of hyaluronic acid. To study the role of the hyaluronic acid capsule in virulence, we derived an acapsular mutant from a mucoid strain of group A Streptococcus by transposon mutagenesis. M protein expression was not altered in the mutant strain. The mucoid wild-type strain grew in fresh human blood and was resistant to phagocytic killing in vitro. In contrast, the acapsular mutant failed to grow in fresh human blood and was sensitive to phagocytic killing in vitro. Loss of capsule was associated with a 100-fold reduction in virulence of the organisms in mice. We conclude that the hyaluronic acid capsule protects mucoid group A streptococci from phagocytosis and has an important role in virulence.

引用

页码：8317 / 8321

页数：5

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