EFFECT OF BORATE ON COLORIMETRIC DETERMINATIONS OF CARBOHYDRATES BY PHENOL-SULFURIC ACID METHOD

被引：27

作者：

LIN, FM

POMERANZ, Y

机构：

[1] Kansas State University, Department of Grain Science and Industry, Manhattan

[2] Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Manhattan

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 24卷 / 01期

关键词：

D O I：

10.1016/0003-2697(68)90067-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Absorbance of solutions containing hexoses is reduced slightly, and of pentoses or pentosans is lowered significantly, by the presence of borate in solutions tested by the phenol-sulfuric acid procedure. The absorbance-lowering effect is related to borate concentration. The presence of borate has no effect on wavelength of maximum absorbance, and lowers height of maximum absorbance by interference with formation of intermediate furan derivatives rather than with chromogen formation. © 1968.

引用

页码：128 / &

共 12 条

[1]

ASHWELL G, 1966, METHODS ENZYMOLOGY, V7, P93

[2]

BARKER JA, 1955, J CHEM SOC, P4276

[3]

COLE EW, 1967, CEREAL CHEM, V44, P411

[4] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].