CHORISMATE SYNTHASE - PRE-STEADY-STATE KINETICS OF PHOSPHATE RELEASE FROM 5-ENOLPYRUVYLSHIKIMATE 3-PHOSPHATE

被引：31

作者：

HAWKES, TR

LEWIS, T

COGGINS, JR

MOUSDALE, DM

LOWE, DJ

THORNELEY, RNF

机构：

[1] UNIV SUSSEX, AFRC, INST PLANT SCI RES, NITROGEN FIXAT LAB, BRIGHTON BN1 9RQ, E SUSSEX, ENGLAND

[2] ICI AGROCHEM, JEALOTTS HILLS RES STN, BRACKNELL RG12 6EY, BERKS, ENGLAND

[3] UNIV GLASGOW, DEPT BIOCHEM, GLASGOW G12 8QQ, SCOTLAND

来源：

BIOCHEMICAL JOURNAL | 1990年 / 265卷 / 03期

关键词：

D O I：

10.1042/bj2650899

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25°C at pH 7.5. No pre-steady-state 'burst' or 'lag' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.

引用

页码：899 / 902

页数：4

共 13 条

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