3D REPRESENTATION OF BIOSTRUCTURES IMAGED WITH AN OPTICAL MICROSCOPE .A. DIGITAL OPTICAL SECTIONING .B. 3D GRAPHIC REPRESENTATION

被引:20
作者
DIASPRO, A
SARTORE, M
NICOLINI, C
机构
[1] Istituto di Biofisica, Universita di Genova, Sestri Ponente, Via Giotto 2-16153, Genova
关键词
3D approximate method; 3D chromatin layout; digital filtering; optical sectioning;
D O I
10.1016/0262-8856(90)90028-4
中图分类号
TP18 [人工智能理论];
学科分类号
081104 ; 0812 ; 0835 ; 1405 ;
摘要
A method for acquiring and processing 3D information from biological specimens imaged with an optical microscope is presented. It is physically based on optical sectioning microscopy, and utilizes a set of digital filters implemented for deblurring optical section images. Rather than the provision of an exact solution for the problem, we have found of more practical use a new approximate method that significantly improves the situation, within reasonable cost parameters. © 1990.
引用
收藏
页码:130 / 141
页数:12
相关论文
共 43 条
[41]  
Weinstein M., 1971, P SOC PHOTOOPT INSTR, V26, P131
[42]  
WEZKA JJ, 1987, IEEE T COMPUT, V23, P125
[43]   AN EVALUATION OF CONFOCAL VERSUS CONVENTIONAL IMAGING OF BIOLOGICAL STRUCTURES BY FLUORESCENCE LIGHT-MICROSCOPY [J].
WHITE, JG ;
AMOS, WB ;
FORDHAM, M .
JOURNAL OF CELL BIOLOGY, 1987, 105 (01) :41-48