DETERMINATION OF THE VITRONECTIN BINDING-SITE ON PLASMINOGEN-ACTIVATOR INHIBITOR-1 (PAI-1)

被引：48

作者：

VANMEIJER, M

GEBBINK, RK

PREISSNER, KT

PANNEKOEK, H

机构：

[1] UNIV AMSTERDAM,ACAD MED CTR,DEPT BIOCHEM K1159,1105 AZ AMSTERDAM,NETHERLANDS

[2] MAX PLANCK GESELL,KERCKHOFF KLIN,HAEMOSTASIS RES UNIT,W-6350 BAD NAUHEIM,GERMANY

来源：

FEBS LETTERS | 1994年 / 352卷 / 03期

关键词：

FIBRINOLYSIS; PAI-1; VITRONECTIN; THROMBIN;

D O I：

10.1016/0014-5793(94)00990-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vitronectin is the carrier protein of plasminogen activator inhibitor 1 (PAI-1). We used a well-characterized panel of anti-human PAI-1 monoclonal antibodies (MoAbs) to localize the vitronectin-binding site on PAI-1. By employing a direct vitronectin/PAI-1 binding assay and two vitronectin-dependent inhibition assays, we demonstrate that the anti-PAI-1 MoAbs CLB-5, CLB-10, CLB-2C8 and 11, directed against different epitopes in the region between amino acids 110 and 145, prevent the interaction of PAI-1 with vitronectin. We conclude that the region between amino acids 110 and 145 of PAI-1 harbours an important determinant for the interaction with vitronectin.

引用

页码：342 / 346

页数：5

共 32 条

[1] PLASMINOGEN-ACTIVATOR INHIBITOR TYPE-1 - REACTIVE CENTER AND AMINO-TERMINAL HETEROGENEITY DETERMINED BY PROTEIN AND CDNA SEQUENCING [J].