DETERMINATION OF THE VITRONECTIN BINDING-SITE ON PLASMINOGEN-ACTIVATOR INHIBITOR-1 (PAI-1)

被引：48

作者：

VANMEIJER, M

GEBBINK, RK

PREISSNER, KT

PANNEKOEK, H

机构：

[1] UNIV AMSTERDAM,ACAD MED CTR,DEPT BIOCHEM K1159,1105 AZ AMSTERDAM,NETHERLANDS

[2] MAX PLANCK GESELL,KERCKHOFF KLIN,HAEMOSTASIS RES UNIT,W-6350 BAD NAUHEIM,GERMANY

来源：

FEBS LETTERS | 1994年 / 352卷 / 03期

关键词：

FIBRINOLYSIS; PAI-1; VITRONECTIN; THROMBIN;

D O I：

10.1016/0014-5793(94)00990-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vitronectin is the carrier protein of plasminogen activator inhibitor 1 (PAI-1). We used a well-characterized panel of anti-human PAI-1 monoclonal antibodies (MoAbs) to localize the vitronectin-binding site on PAI-1. By employing a direct vitronectin/PAI-1 binding assay and two vitronectin-dependent inhibition assays, we demonstrate that the anti-PAI-1 MoAbs CLB-5, CLB-10, CLB-2C8 and 11, directed against different epitopes in the region between amino acids 110 and 145, prevent the interaction of PAI-1 with vitronectin. We conclude that the region between amino acids 110 and 145 of PAI-1 harbours an important determinant for the interaction with vitronectin.

引用

页码：342 / 346

页数：5

共 32 条

[11]

HARLOW E, 1988, ANTIBODIES LABORATOR

[12]

HEKMAN CM, 1985, J BIOL CHEM, V260, P11581

[13] IMPLICATIONS OF THE 3-DIMENSIONAL STRUCTURE OF ALPHA-1-ANTITRYPSIN FOR STRUCTURE AND FUNCTION OF SERPINS [J].

HUBER, R ;

CARRELL, RW .

BIOCHEMISTRY, 1989, 28 (23) :8951-8966

[14]

KEIJER J, 1991, BLOOD, V78, P401

[15]

KEIJER J, 1991, BLOOD, V78, P1254

[16]

KEIJER J, 1991, J BIOL CHEM, V266, P10700

[17]

LAWRENCE DA, 1994, J BIOL CHEM, V269, P15223

[18]

MIMURO J, 1989, J BIOL CHEM, V264, P936

[19] STRUCTURAL BASIS OF LATENCY IN PLASMINOGEN-ACTIVATOR INHIBITOR-1 [J].

MOTTONEN, J ;

STRAND, A ;

SYMERSKY, J ;

SWEET, RM ;

DANLEY, DE ;

GEOGHEGAN, KF ;

GERARD, RD ;

GOLDSMITH, EJ .

NATURE, 1992, 355 (6357) :270-273

[20]

MY T, 1986, P NATL ACAD SCI USA, V83, P6776

← 1 2 3 4 →