LOCALIZATION OF METASTASIS SUPPRESSOR GENE(S) FOR RAT PROSTATIC-CANCER TO THE LONG ARM OF HUMAN-CHROMOSOME-10

被引：55

作者：

NIHEI, N

ICHIKAWA, T

KAWANA, Y

KURAMOCHI, H

KUGO, H

OSHIMURA, M

KILLARY, AM

RINKERSCHAEFFER, CW

BARRETT, JC

ISAACS, JT

SHIMAZAKI, J

机构：

[1] CHIBA UNIV,SCH MED,DEPT UROL,CHUO KU,CHIBA 260,JAPAN

[2] TOTTORI UNIV,SCH MED,DEPT MOLEC & CELL GENET,YONAGO,TOTTORI 683,JAPAN

[3] UNIV TEXAS,MD ANDERSON CANCER CTR,DIV LAB MED,HEMATOPATHOL PROGRAM,HOUSTON,TX 77030

[4] JOHNS HOPKINS UNIV,SCH MED,JOHNS HOPKINS ONCOL CTR,BALTIMORE,MD 21205

[5] NIEHS,ENVIRONM CARCINOGENESIS PROGRAM,MOLEC CARCINOGENESIS LAB,RES TRIANGLE PK,NC 27709

来源：

GENES CHROMOSOMES & CANCER | 1995年 / 14卷 / 02期

关键词：

D O I：

10.1002/gcc.2870140205

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To examine the role of human chromosome 10 in development of prostatic cancer, we introduced human chromosome 10 into highly metastatic rat prostatic cancer cells by microcell-mediated chromosome transfer. Microcell hybrid cells introduced with human chromosome 10 showed suppression of the metastatic ability to the lung to some extent without any suppression of tumorigenicity, although the tumor growth rate decreased slightly. To minimize the region that contains metastasis suppressive activity, the hybrid cells in metastasis foci of lung were established in culture and reanalyzed for portions of human chromosome 10 retained in the metastasis tissues. Cytogenetic and molecular analyses demonstrated that loss of the region between 10cen and D10S215 on human chromosome arm 10q was related to expression of the metastatic phenotype. These results demonstrate that the region between 10cen and D10S215 on human chromosome arm 10q contains at least one of the metastasis suppressor genes for rat prostatic cancer. (C) 1995 Wiley-Liss, Inc.

引用

页码：112 / 119

页数：8