STUDIES ON CEPHALOPOD RHODOPSIN - FATTY-ACID ESTERS OF SUCROSE AS EFFECTIVE DETERGENTS

被引：38

作者：

NASHIMA, K ^{[1
]}

MITSUDO, M ^{[1
]}

KITO, Y ^{[1
]}

机构：

[1] OSAKA KYOIKU UNIV, DEPT HLTH SCI, HIRANO, OSAKA 547, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 536卷 / 01期

关键词：

D O I：

10.1016/0005-2795(78)90053-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Squid rhodopsin was extracted with solutions of fatty acid esters of sucrose (monolaurate and monostearate) and purified by DEAE-cellulose and concanavalin A-Sepharose affinity chromatography. The purified rhodopsin ( A280 A480 = 2.5) contained 2.3 mol of glucosamine and 1.2 mol of phospholipid per mol of rhodopsin. The photoproduct metarhodopsin was also stable in these detergent solutions as in digitonin solution. Concanavalin A had no affinity for retinochrome. © 1978.

引用

页码：78 / 87

页数：10

共 21 条

[1] BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[2] DETERMINATION OF SECONDARY STRUCTURES OF PROTEINS BY CIRCULAR-DICHROISM AND OPTICAL ROTATORY DISPERSION
CHEN, YH
YANG, JT
MARTINEZ, HM
[J]. BIOCHEMISTRY, 1972, 11 (22) : 4120 - +
[3] HAGINS FM, 1973, J BIOL CHEM, V248, P3298
[4] ELECTRICAL SIGNS OF INFORMATION FLOW IN PHOTORECEPTORS
HAGINS, WA
[J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1965, 30 : 403 - &
[5] AMINO-TERMINAL TRYPTIC PEPTIDE OF BOVINE RHODOPSIN - GLYCOPEPTIDE CONTAINING 2 SITES OF OLIGOSACCHARIDE ATTACHMENT
HARGRAVE, PA
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 492 (01) : 83 - 94
[6] STRUCTURE OF VISUAL PIGMENTS .I. PURIFICATION MOLECULAR WEIGHT AND COMPOSITION OF BOVINE VISUAL PIGMENT500
HELLER, J
[J]. BIOCHEMISTRY, 1968, 7 (08) : 2906 - &
[7] THE RHODOPSIN SYSTEM OF THE SQUID
HUBBARD, R
STGEORGE, RCC
[J]. JOURNAL OF GENERAL PHYSIOLOGY, 1958, 41 (03) : 501 - 528
[8] KITO Y, 1973, NATURE-NEW BIOL, V243, P53
[9] NASHIMA K, 1977, 16TH P ANN C BIOPH S, P93
[10] METHODS OF PREPARATION - FATTY ACID ESTERS OF SUCROSE
OSIPOW, L
SNELL, FD
YORK, WC
FINCHLER, A
[J]. INDUSTRIAL AND ENGINEERING CHEMISTRY, 1956, 48 (09): : 1459 - 1462

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