INTERACTION OF METALLOCHROMIC INDICATORS WITH CALCIUM SEQUESTERING ORGANELLES

被引：14

作者：

OHNISHI, ST ^{[1
]}

机构：

[1] HAHNEMANN MED COLL & HOSP,DEPT BIOL CHEM,PHILADELPHIA,PA 19102

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 585卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Arsenazo III; Ca[!sup]2+[!/sup] sequestration; Ca[!sup]2+[!/sup] transport; Cotransport; Metallochromic indicator; Murexide;

D O I：

10.1016/0304-4165(79)90031-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Examples are presented of the interaction between cell organelles and metallochromic indicators used in the measurement of ionized Ca2+. Sarcoplasmic reticulum was found to sequester murexide type indicators along with Ca2+ in the presence of ATP, but not to sequester arsenazo III and antipyrylazo III. The presence of a permeable anion suppresses the sequestration of murexide type indicators by the sarcoplasmic reticulum. In the presence of ruthenium red, both rat liver and beef heart mitochondria release sequestered Ca2+ with arsenazo III, but not with murexide. © 1979.

引用

页码：315 / 319

页数：5

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