INTERACTION OF METALLOCHROMIC INDICATORS WITH CALCIUM SEQUESTERING ORGANELLES

被引：14

作者：

OHNISHI, ST ^{[1
]}

机构：

[1] HAHNEMANN MED COLL & HOSP,DEPT BIOL CHEM,PHILADELPHIA,PA 19102

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 585卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Arsenazo III; Ca[!sup]2+[!/sup] sequestration; Ca[!sup]2+[!/sup] transport; Cotransport; Metallochromic indicator; Murexide;

D O I：

10.1016/0304-4165(79)90031-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Examples are presented of the interaction between cell organelles and metallochromic indicators used in the measurement of ionized Ca2+. Sarcoplasmic reticulum was found to sequester murexide type indicators along with Ca2+ in the presence of ATP, but not to sequester arsenazo III and antipyrylazo III. The presence of a permeable anion suppresses the sequestration of murexide type indicators by the sarcoplasmic reticulum. In the presence of ruthenium red, both rat liver and beef heart mitochondria release sequestered Ca2+ with arsenazo III, but not with murexide. © 1979.

引用

页码：315 / 319

页数：5

共 16 条

[11] VELOCITY OF CALCIUM BINDING OF ISOLATED SARCOPLASMIC RETICULUM [J].

OHNISHI, T ;

EBASHI, S .

JOURNAL OF BIOCHEMISTRY, 1964, 55 (06) :599-&

[12] ANALYSIS OF A CALCIUM-ION BINDING SYSTEM COMPOSED OF 2 DIFFERENT SITES [J].

OHNISHI, T ;

YU, BP ;

MASORO, EJ ;

BERTRAND, HA .

BIOPHYSICAL JOURNAL, 1972, 12 (10) :1251-&

[13] ANTIPYRYLAZO-III, A MIDDLE RANGE CA2+ METALLOCHROMIC INDICATOR [J].

SCARPA, A ;

BRINLEY, FJ ;

DUBYAK, G .

BIOCHEMISTRY, 1978, 17 (08) :1378-1386

[14]

SCARPA A, 1976, BIOCH MEMBRANE TRANS, P552

[15]

SCHNEIDER WC, 1957, MANOMETRIC TECHNIQUE, P188

[16]

SORDAHL LA, 1967, MOL PHARMACOL, V3, P509

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