MINIMAL MODEL ANALYZING RESPONSE OF GLYCINE RECEPTORS EXPRESSED IN XENOPUS OOCYTE - INHIBITION BY A LIPID HYDROPEROXIDE

被引:6
作者
AOSHIMA, H [1 ]
INOUE, Y [1 ]
UEDA, E [1 ]
KITAGAWA, M [1 ]
NISHINO, T [1 ]
机构
[1] YAMAGUCHI UNIV,FAC AGR,DEPT BIOL SCI,YAMAGUCHI 753,JAPAN
关键词
D O I
10.1093/oxfordjournals.jbchem.a123790
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Glycine receptor (GlyR) was expressed in Xenopus oocytes by injecting rat brain mRNA. Glycine (Gly)-elicited responses in the oocyte were measured by the voltage-clamping method. The following measurements were made to establish the relationship between Gly concentration and the current: 1) Gly-induced membrane current before desensitization, 2) Gly-induced membrane current after desensitization equilibrium, 3) fraction of the active form of the receptor after desensitization equilibrium, 4) rate of recovery of the desensitized receptors upon removal of Gly. These results were analyzed on the basis of the minimal model proposed for nicotinic acetylcholine and gamma-aminobutyric acid A receptor. The equilibrium and rate constants of the model were evaluated for GlyR. The effects of procaine and 13-L-hydroperoxylinoleic acid (LOOH) on GlyR were examined electrophysiologically. LOOH noncompetitively inhibited the receptor with the inhibition constant of 27-mu-M, while 1 mM procaine, a local anesthetic, did not inhibit GlyR at all.
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收藏
页码:523 / 528
页数:6
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