PURIFICATION OF A ROTENONE-INSENSITIVE NAD(P)H DEHYDROGENASE FROM THE INNER SURFACE OF THE INNER MEMBRANE OF RED BEETROOT MITOCHONDRIA

被引：30

作者：

RASMUSSON, AG ^{[1
]}

FREDLUND, KM ^{[1
]}

MOLLER, IM ^{[1
]}

机构：

[1] UNIV LUND,DEPT PLANT PHYSIOL,BOX 7007,S-22007 LUND 7,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1141卷 / 01期

关键词：

MITOCHONDRION; NAD(P)H DEHYDROGENASE; INNER MEMBRANE; ENZYME PURIFICATION; PROTEIN PURIFICATION; RESPIRATORY CHAIN; ROTENONE INSENSITIVITY; (PLANT);

D O I：

10.1016/0005-2728(93)90196-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The soluble fraction of disrupted red beetroot mitochondria was resolved by anion-exchange chromatography. Three NADH-oxidising activities were found, including one duroquinone reductase oxidising both NADH and NADPH. This NAD(P)H-duroquinone reductase, which we assign as the internal rotenone-insensitive NAD(P)H dehydrogenase, was further purified by affinity chromatography into 26 kDa polypeptide.

引用

页码：107 / 110

页数：4

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