INVESTIGATION OF THE PSEUDOMONAS-AERUGINOSA-AMPR GENE AND ITS ROLE AT THE CHROMOSOMAL AMPC BETA-LACTAMASE PROMOTER

被引：40

作者：

LODGE, J

BUSBY, S

PIDDOCK, L

机构：

[1] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

[2] UNIV BIRMINGHAM,SCH MED,DEPT INFECT,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 111卷 / 2-3期

关键词：

PSEUDOMONAS-AERUGINOSA; RECOMBINANT DNA; BETA-LACTAMASE EXPRESSION; AMPR SEQUENCE; DNA BINDING ASSAY;

D O I：

10.1016/0378-1097(93)90399-M

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The complete sequence of the Pseudomonas aeruginosa beta-lactamase regulator gene ampR has been determined. The amino acid sequence encoded by this gene is compared with other members of the LysR family of transcription factors. The P. aeruginosa ampC promoter was subcloned and gel mobility shift assays were used to demonstrate specific binding of AmpR to the ampC promoter region.

引用

页码：315 / 319

页数：5