METHODS FOR MEASUREMENT OF FATTY-ACID AND CHOLESTEROL-METABOLISM

被引：33

作者：

HELLERSTEIN, MK

机构：

[1] UNIV CALIF BERKELEY, DEPT NUTR SCI, BERKELEY, CA 94720 USA

[2] UNIV CALIF SAN FRANCISCO, SAN FRANCISCO GEN HOSP, DEPT MED, DIV ENDOCRINOL & METAB, SAN FRANCISCO, CA 94110 USA

来源：

CURRENT OPINION IN LIPIDOLOGY | 1995年 / 6卷 / 03期

关键词：

D O I：

10.1097/00041433-199506000-00010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The measurement of dynamic fluxes of lipids (biosynthesis, oxidation, and intermediary metabolism) poses difficult challenges. Two fundamental advances have been made recently for measuring the biosynthesis of lipids, namely mass isotopomer distribution analysis and labeled water incorporation. These techniques have resolved the central methodologic problem in biosynthesis by establishing the true precursor isotope enrichment. Mass isotopomer distribution analysis also permits uncontaminated pulse-chase decay curves and intracellular precursor fluxes to be measured. In contrast, the isotopic measurement of lipid oxidation rates remains unreliable because of the wide variability in the recovery of labeled carbon dioxide.

引用

页码：172 / 181

页数：10

共 68 条

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