METHODS FOR MEASUREMENT OF FATTY-ACID AND CHOLESTEROL-METABOLISM

被引：33

作者：

HELLERSTEIN, MK

机构：

[1] UNIV CALIF BERKELEY, DEPT NUTR SCI, BERKELEY, CA 94720 USA

[2] UNIV CALIF SAN FRANCISCO, SAN FRANCISCO GEN HOSP, DEPT MED, DIV ENDOCRINOL & METAB, SAN FRANCISCO, CA 94110 USA

来源：

CURRENT OPINION IN LIPIDOLOGY | 1995年 / 6卷 / 03期

关键词：

D O I：

10.1097/00041433-199506000-00010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The measurement of dynamic fluxes of lipids (biosynthesis, oxidation, and intermediary metabolism) poses difficult challenges. Two fundamental advances have been made recently for measuring the biosynthesis of lipids, namely mass isotopomer distribution analysis and labeled water incorporation. These techniques have resolved the central methodologic problem in biosynthesis by establishing the true precursor isotope enrichment. Mass isotopomer distribution analysis also permits uncontaminated pulse-chase decay curves and intracellular precursor fluxes to be measured. In contrast, the isotopic measurement of lipid oxidation rates remains unreliable because of the wide variability in the recovery of labeled carbon dioxide.

引用

页码：172 / 181

页数：10

共 68 条

[51]

Rutler P.C., Kryshak E.J., Marsh M., Rizza R.A., Effect of insulin on oxidation of intracellularly- and extracellularly-derived glucose in patients with NIDDM. Evidence for primary defect in glucose transport and/or phosphorylation but not oxidation, Diabetes, 39, pp. 1373-1380, (1990)

[52]

Waterlow J.C., Garlick P.J., Millward D.J., Protein Turnover in Mammalian Tissues and in the Whole Body, (1978)

[53]

Klein S., Young V.R., Blackburn C.L.A., Bistnan B.R., Wolfe R.R., Palmitate and glycerol kinetics during brief starvation in normal weight young adult and elderly subjects, J Clin Invest, 78, pp. 928-933, (1986)

[54]

Wolfe R.R., Herndon D.N., Jahoor F., Miyoshi H., Wolfe M., Effect of severe burn injury on substrate cycling by glucose and fatty acids, N Engl J Med, 317, pp. 403-408, (1987)

[55]

Wolfe R.R., Klein S., Carraro F., Weber J.M., Role of triglyceridefatty acid cycle in controlling fat metabolism in humans during and after exercise, Am J Physiol, 258, (1990)

[56]

Campbell P., Carlson N.G., Nurjhan N., Fat metabolism in human obesity, Am J Physiol, 266, (1994)

[57]

Wolfe R.R., Klein S., Carraro F., Weber J.M., Role of triglyceridefatty acid cycling in controlling fat metabolism in humans during and after exercise, Am J Physiol, 258, (1990)

[58]

Hellerstein M.K., Benowitz N.L., Neese R.A., Hoh R., Jacob P., Fong I., Hsieh J., Faix D., Effects of cigarette smoking and its cessation on lipid metabolism and energy expenditure in heavy smokers, J Clin Invest, 93, pp. 265-272, (1994)

[59]

Hellerstein M.K., Neese R.A., Schwarz J.-M., Measurement of absolute rates of hepatic de-novo lipogenesis and reesterification of free fatty acids in humans, Am J Physiol, 265, (1993)

[60]

Dagenais G.R., Tancredi R.G., Zierler K.L., Free fatty acid oxidation by forearm muscle at rest and evidence for an intramuscular lipid pool in the human forearm, J Clin Invest, 58, pp. 421-431, (1976)

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