DNA-SEQUENCE OF A FIBROBACTER-SUCCINOGENES MIXED-LINKAGE BETA-GLUCANASE (1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE) GENE

被引:75
作者
TEATHER, RM
ERFLE, JD
机构
[1] Animal Research Centre, Research Branch, Agriculture Canada, Ottawa
关键词
D O I
10.1128/jb.172.7.3837-3841.1990
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The DNA sequence of a mixed-linkage β-glucanase (1,3-1,4-β-D-glucan 4-glucanohydrolase [EC 3.2.1.73]) gene from Fibrobacter succinogenes cloned in Escherichia coli was determined. The general features of this gene are very similar to the consensus features for other gram-negative bacterial genes. The gene product was processed for export in E. coli. There is a high level of sequence homology between the structure of this glucanase and the structure of a mixed-linkage β-glucanase from Bacillus subtilis. The nonhomologous region of the amino acid sequence includes a serine-rich region containing five repeats of the sequence Pro-Xxx-Ser-Ser-Ser-Ser(Ala or Val) which may be functionally related to the serine-rich region observed in Pseudomonas fluorescens cellulase and the serine- and/or threonine-rich regions observed in Cellulomonas fimi endoglucanase and exoglucanase, in Clostridium thermocellum endoglucanases A and B, and in Trichoderma reesei cellobiohydrolase I, cellobiohydrolase II, and endoglucanase I.
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页码:3837 / 3841
页数:5
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