NUCLEOTIDE-SEQUENCE OF THE ORIGIN OF REPLICATION OF THE ESCHERICHIA-COLI K-12 CHROMOSOME

被引：209

作者：

MEIJER, M

BECK, E

HANSEN, FG

BERGMANS, HEN

MESSER, W

VONMEYENBURG, K

SCHALLER, H

机构：

[1] MAX PLANCK INST MOLEC GENET, D-1000 BERLIN 33, FED REP GER

[2] UNIV HEIDELBERG, D-6900 HEIDELBERG 1, FED REP GER

[3] UNIV COPENHAGEN, INST MICROBIOL, COPENHAGEN, DENMARK

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 02期

关键词：

D O I：

10.1073/pnas.76.2.580

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The origin of replication, oriC, of the E. coli chromosome was mapped within a DNA segment of 422 base-pairs. The nucleotide sequence of this segment was determined. The source of DNA for the sequence analysis was a minichromosome constructed in vivo, consisting exclusively of chromosomal DNA and a minichromosome constructed by cloning in vitro. The nucleotide sequence of the replication origin is characterized by a high degree of repetitiveness due to both inverted and direct repeats. Sequence homologies were found between portions of the replication origins of E. coli and phages .lambda. and G4. This suggests similarities in some steps in the initiation of replication of the different replicons.

引用

页码：580 / 584

页数：5

共 29 条

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