NUCLEOTIDE-SEQUENCE OF THE RAW-STARCH-DIGESTING AMYLASE GENE FROM BACILLUS SP B1018 AND ITS STRONG HOMOLOGY TO THE CYCLODEXTRIN GLUCANOTRANSFERASE GENES

被引：36

作者：

ITKOR, P ^{[1
]}

TSUKAGOSHI, N ^{[1
]}

UDAKA, S ^{[1
]}

机构：

[1] NAGOYA UNIV,FAC AGR,CHIKUSA,NAGOYA 46401,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90855-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene coding for the raw-starch-digesting amylase from Bacillus sp. B1018 was cloned into Escherichia coli, and its nucleotide sequence was determined. Starting at an ATG codon, there was an open reading frame composed of 2139 bp (713 amino acids). The NH2-terminal portion encoded a 27 amino acid-long signal peptide. S1 mapping revealed the presence of a typical promoter region upstream from the transcription initiation site. The deduced amino acid sequence of the extracellular mature enzyme was very similar to those of cyclodextrin glucanotransferases (CGTases), especially that of alkalophilic Bacillus sp. 1011 (87% homology). In fact, the B1018 amylase showed CGTase activity. The COOH-terminal portion of the B1018 amylase shows significant homology with other raw-starch-digesting enzymes. © 1990.

引用

页码：630 / 636

页数：7

共 20 条

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