PARTIAL-PURIFICATION AND CHARACTERIZATION OF A DNA HELICASE FROM CHLOROPLASTS OF GLYCINE-MAX

被引：15

作者：

CANNON, GC

HEINHORST, S

机构：

[1] Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, 39406, MS

来源：

PLANT MOLECULAR BIOLOGY | 1990年 / 15卷 / 03期

关键词：

chloroplasts; DNA helicase; DNA replication;

D O I：

10.1007/BF00019162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg2+ or Mn2+. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure. © 1990 Kluwer Academic Publishers.

引用

页码：457 / 464

页数：8

共 21 条

[11] CHLOROPLAST AND MITOCHONDRIAL-DNA POLYMERASES FROM CULTURED SOYBEAN CELLS [J].