FUNCTIONAL-ANALYSIS IN YEAST OF CDNA CODING FOR THE MITOCHONDRIAL RIESKE IRON-SULFUR PROTEIN OF HIGHER-PLANTS

被引:17
作者
HUANG, J [1 ]
STRUCK, F [1 ]
MATZINGER, DF [1 ]
LEVINGS, CS [1 ]
机构
[1] N CAROLINA STATE UNIV,DEPT GENET,BOX 7614,RALEIGH,NC 27695
关键词
POLYMERASE CHAIN REACTION; COMPLEMENTATION; ZEA-MAYS; NICOTIANA-TABACUM; COMPLEX-III;
D O I
10.1073/pnas.88.23.10716
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
cDNA clones coding for the nuclear-encoded mitochondrial Rieske iron-sulfur protein (RISP) have been isolated from maize and tobacco. Complementation analysis of hybrid proteins consisting of different domains of plant and yeast RISPs showed that the carboxyl two-thirds of the plant protein is functionally equivalent to that of the yeast protein. The amino terminus of the RISP, however, seems to be species specific because this region is not interchangeable between plant and yeast proteins. Complementation analysis of hybrid proteins also identified a structurally conserved domain probably essential for the function of bc1 complex RISPs. A specific domain from the plant RISP was found to cause temperature-sensitive respiratory growth in yeast. We have demonstrated that yeast can serve as a model system to study the structural and functional relationships of plant gene products that are enzymatic components of the mitochondrial respiratory chain.
引用
收藏
页码:10716 / 10720
页数:5
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