FUNCTIONAL-ANALYSIS IN YEAST OF CDNA CODING FOR THE MITOCHONDRIAL RIESKE IRON-SULFUR PROTEIN OF HIGHER-PLANTS

被引：17

作者：

HUANG, J ^{[1
]}

STRUCK, F ^{[1
]}

MATZINGER, DF ^{[1
]}

LEVINGS, CS ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV,DEPT GENET,BOX 7614,RALEIGH,NC 27695

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 23期

关键词：

POLYMERASE CHAIN REACTION; COMPLEMENTATION; ZEA-MAYS; NICOTIANA-TABACUM; COMPLEX-III;

D O I：

10.1073/pnas.88.23.10716

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

cDNA clones coding for the nuclear-encoded mitochondrial Rieske iron-sulfur protein (RISP) have been isolated from maize and tobacco. Complementation analysis of hybrid proteins consisting of different domains of plant and yeast RISPs showed that the carboxyl two-thirds of the plant protein is functionally equivalent to that of the yeast protein. The amino terminus of the RISP, however, seems to be species specific because this region is not interchangeable between plant and yeast proteins. Complementation analysis of hybrid proteins also identified a structurally conserved domain probably essential for the function of bc1 complex RISPs. A specific domain from the plant RISP was found to cause temperature-sensitive respiratory growth in yeast. We have demonstrated that yeast can serve as a model system to study the structural and functional relationships of plant gene products that are enzymatic components of the mitochondrial respiratory chain.

引用

页码：10716 / 10720

页数：5

共 48 条

[31] SPECIFIC ENZYMATIC AMPLIFICATION OF DNA INVITRO - THE POLYMERASE CHAIN-REACTION [J].

MULLIS, K ;

FALOONA, F ;

SCHARF, S ;

SAIKI, R ;

HORN, G ;

ERLICH, H .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1986, 51 :263-273

[32] CLONING AND SEQUENCE-ANALYSIS OF A CDNA-ENCODING THE RIESKE IRON-SULFUR PROTEIN OF RAT MITOCHONDRIAL CYTOCHROME BC1 COMPLEX [J].

NISHIKIMI, M ;

HOSOKAWA, Y ;

TODA, H ;

SUZUKI, H ;

OZAWA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 159 (01) :19-25

[33] COMPOSITION, STRUCTURE, AND FUNCTION OF COMPLEX III OF RESPIRATORY-CHAIN [J].

RIESKE, JS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 456 (02) :195-243

[34]

RIESKE JS, 1967, METHOD ENZYMOL, V10, P357

[35] ENZYMATIC AMPLIFICATION OF BETA-GLOBIN GENOMIC SEQUENCES AND RESTRICTION SITE ANALYSIS FOR DIAGNOSIS OF SICKLE-CELL ANEMIA [J].

SAIKI, RK ;

SCHARF, S ;

FALOONA, F ;

MULLIS, KB ;

HORN, GT ;

ERLICH, HA ;

ARNHEIM, N .

SCIENCE, 1985, 230 (4732) :1350-1354

[36]

Sambrook J., 1989, MOL CLONING LAB MANU

[37] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[38] ISOLATION AND AMINO-ACID-SEQUENCE OF THE RIESKE IRON SULFUR PROTEIN OF BEEF-HEART UBIQUINOL - CYTOCHROME-C REDUCTASE [J].

SCHAGGER, H ;

BORCHART, U ;

MACHLEIDT, W ;

LINK, TA ;

VONJAGOW, G .

FEBS LETTERS, 1987, 219 (01) :161-168

[39] HIGH-EFFICIENCY TRANSFORMATION OF INTACT YEAST-CELLS USING SINGLE STRANDED NUCLEIC-ACIDS AS A CARRIER [J].

SCHIESTL, RH ;

GIETZ, RD .

CURRENT GENETICS, 1989, 16 (5-6) :339-346

[40] A YEAST MITOCHONDRIAL PRESEQUENCE FUNCTIONS AS A SIGNAL FOR TARGETING TO PLANT-MITOCHONDRIA INVIVO [J].

SCHMITZ, UK ;

LONSDALE, DM .

PLANT CELL, 1989, 1 (08) :783-791

← 1 2 3 4 5 →